U ovom radu ispitana je pogodnost četiri komercijalne selektivne i diferencijalne krute hranjive podloge (EC X-Gluc, Endo, Mac Conkey i m-Faecal Coliform agara proizvođača Biolife) za kultivaciju ukupnih koliformnih bakterija iz uzoraka površinskih voda. Određeni broj ukupnih koliformnih bakterija i postotni udio bakterije Escherichia coli u populaciji ukupnih koliforma u istom uzorku bio je značajno različit na različitim hranjivim podlogama. U 6 od 19 ispitivanih uzoraka vode varijacija određenog broja ukupnih koliformnih bakterija rezultirala je različitom interpretacijom kvalitete vode. Kod izbora komercijalno dostupne hranjive podloge za uzgoj ukupnih koliformnih bakterija potrebno je dobro analizirati sve pozitivne i negativne strane pojedine hranjive podloge. EC X-Gluc agar je prema svom kvalitativnom sastavu visoko selektivna i diferencijalna kromogena podloga koja se može preporučiti za uzgoj koliformnih bakterija u odnosu na preostale tri podloge čija je primjenjivost ispitivana u ovom istraživanju..; This paper investigates the suitability of four commercial selective, differential nutrient media (EC X-Gluc, Endo, Mac Conkey and m-Faecal Coliform agar manufactured by Biolife) for cultivation of total coliform bacteria from surface water samples. The number of total coliform bacteria and the percentage of the bacteria Escherichia coli in the total coliform population in the same sample were significantly different on different nutrient media.In 6 of 19 tested water samples, a variation in the number of total coliform bacteria resulted in different interpretations of water quality. When selecting a commercially available nutrient media for cultivation of total coliform bacteria, it is necessary to perform an adequate analysis of all positive and negative aspects of individual nutrient media. The EC X-Gluc agar is, according to its quality composition, a highly selective, differential chromogenic medium which is more recommendable for cultivation of coliform bacteria than the remaining three media whose suitability was tested during this investigation.
